Assessment of Genetic and Epigenetic Variations in Potato Somatic Hybrids by Methylation-sensitive Issr and Rapd Markers
نویسندگان
چکیده
Genetic and epigenetic changes (DNA methylation) in the mother plants of somatic hybrids (1st cycle) and their regenerants (30th cycles sub-cultured) were analyzed using methylation-sensitive ISSR and RAPD markers. ISSR and RAPD profiles and their cluster analyses based on the Jaccard’s similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. While for the epigenetic changes in the samples, the MS-ISSR markers detected DNA methylation (2.7%) in the regenerants compared to the mother plant (1%), whereas MS-RAPD markers detected a little methylation patterns in the regeneratns (0.6%) compared to the mother plants (0.4%).
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